The aim of this practical is to carry out TLC in which the substances we wish to separate are absorbed onto the thin layer. Lipids are determined by isolation and the ability to purify the substance. Due to the way the substances interact with the matrix in different ways we are able to seperate them. Substances which interact strongly with the matrix but not with the solvent will move very slowly and those soluble in the solvent will dissolve easily and be carried along the solvent.
Results:
Egg yolk contains trioleine, cholesterol palmitate and phosphatidylethonolamine, The most polar of the three elements is the second element. It hardly moves up the TLC because of the presence of several polar groups; phosphate groups, amine groups and several oxygens , and cholesterol palmitate being the most non polar.
This is great - we featured this and have a mini-application note available at our blog - http://www.blog.analtech.com/blog/bid/19925/Separation-of-Lipids-via-Thin-Layer-Chromatography
ReplyDeleteHmm, you have been discovered by a TLC chemist! I should note however, that the picture is of a paper chromatography of animo acids, rather than a TLC of lipids.
ReplyDeleteIN your discussion you incorrectly ID the most polar substance. The phosphatidylethanolamine is the most polar as it moves the least - the leas poar (i.e. most hydrophobic) will move the most.
/steve
The tips that you shared on the blog regarding the network security and all are useful and I am thanking you for sharing it. Good luck medicine shoppe gulf canada square
ReplyDelete